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  • Improved methods for mass production of magnetosomes and applications . . .
    Basit et al Microb Cell Fact Page7of11 [37] Applicationsofmagnetosomesreecttheirdis- tribution,ferrimagneticproperties,nanoscalesize, membrane-boundstructure,anddispersalability
  • RESEARCH Open Access
    Abstract Background Aspergillus niger is widely used in industrial enzyme production due to its strong secretion capacity and the status of generally recognized as safe (GRAS) However, heterologous protein expression in A niger is frequently constrained by high levels of background endogenous protein secretion, limited access to native high transcription loci, and limitations in the
  • Whole-cell biocatalysts by design
    Abstract Whole-cell biocatalysts provide unique advantages and have been widely used for the eficient biosynthesis of value-added fine and bulk chemicals, as well as pharmaceutically active ingredients What is more, advances in synthetic biology and metabolic engineering, together with the rapid development of molecular genetic tools, have brought about a renaissance of whole-cell
  • RESEARCH Open Access
    herichia coli cells Microb Ce TLR4 NF-κB pathway Biomed Pharmacoth comparison with LPS Allerg Th17 cell responses Front Imm G, Zhou P, Chung HS Metabolic engineering of Escherichia coli to produce a mono-phospho yl lipid a adjuvant Metab En lla CR, Mitchell TC The vaccine adjuvant monophosphoryl lipid A as a TRIF-bi sed agonist of TLR4
  • Dual regulation of lipid droplet-triacylglycerol metabolism and ERG9 . . .
    Background: The limitation of storage space, product cytotoxicity and the competition for precursor are the major challenges for eficiently overproducing carotenoid in engineered non‐carotenogenic microorganisms In this work, to improve β‐carotene accumulation in Saccharomyces cerevisiae, a strategy that simultaneous increases cell storage capability and strengthens metabolic flux to
  • Enhancement of astaxanthin production in Xanthophyllomyces dendrorhous . . .
    Microb Cell Fact 2014;13:175 9 Gassel S, Schewe H, Schmidt I, Schrader J, Sandmann G Multiple improvement of astaxanthin biosynthesis in Xanthophyllomyces den-drorhous by a combination of conventional mutagenesis and metabolic pathway engineering Biotechnol Lett 2013;35:565–9 10
  • Microbial Cell Factories - BioMed Central
    Basic and applied studies of Metabolic Transcription Anal-ysis (MTA) may be valuable, because have the potential to: 1) determine transcriptional responses to the genetic modifications introduced into the organism during the process of genetic engineering, providing a deep insight on the physiological mechanisms of the cell; 2) identify
  • Engineering of daidzein 3’-hydroxylase P450 enzyme into catalytically . . .
    Abstract A cytochrome P450 (CYP) enzyme, 3’-daidzein hydroxylase, CYP105D7 (3’-DH), responsible for daidzein hydroxylation at the 3’-position, was recently reported CYP105D7 (3’-DH) is a class I type of CYP that requires electrons provided through electron transfer proteins such as ferredoxin and ferredoxin reductase Presently, we constructed an artificial CYP in order to develop a
  • Development of an efficient protein expression system in the . . .
    Background The development of eficient, high-yield and low-cost protein production platforms has received increasing attention in recent years Due to their excellent protein secretion capacity, filamentous fungi have been exten-sively studied and engineered for the production of a wide range of valuable proteins, including enzymes, pharmaceutical proteins, and industrial chemicals The





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